FIG 5.

LINC01094 contributed to the development of ccRCC by sponging miR-224-5p to regulate CHSY1 expression. (A) After transfection with empty vector pcDNA3.1 or pcDNA3.1/CHSY1 plasmid, RT-qPCR was used to estimate CHSY1 expression in 769-P cells (**, P < 0.01 [Student t test]). (B and C) The function of LINC01094/miR-224-5p/CHSY1 in proliferation was determined using CCK-8 (*, P < 0.05 [2-way ANOVA]) and EdU (**, P < 0.01 [two-way or one-way ANOVA]) assays. (D) The migratory capability was evaluated with a transwell assay (***, P < 0.001 [one-way ANOVA]). (E and F) Western blotting was used to measure EMT-relevant proteins. n.s., not significant.