FIG 2.

H19 promoted the expression of BRD4 by binding to miR-152-3p. (A) The expression levels of H19, miR-152-3p, and BRD4 were detected by qRT-PCR in MM1.R and RPMI-8226 cells transfected with scramble vector (shNC) or sh-H19 vector. (B) The expression of miR-152-3p and BRD4 was detected by qRT-PCR in MM1.R and RPMI-8226 cells transfected with miR-152-3p mimics and inhibitor. The potential binding site in the sequences of H19 with miR-152-3p (C) and miR-152-3p with the 3′-UTR of BRD4 (D) was analyzed through bioinformatics. A dual luciferase activity assay was implemented to confirm the binding of H19/miR-152-3p (E) and miR-152-3p/BRD4 mRNA (F). All data are representative of 3 independent experiments. *, P < 0.05; **, P < 0.01.