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. 2020 Jan 10;10:1526. doi: 10.3389/fphys.2019.01526

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Copyright © 2020 Emig, Zgierski-Johnston, Beyersdorf, Rylski, Ravens, Weber, Kohl, Hörner and Peyronnet.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Area of human primary atrial fibroblasts after 4 days of culture on CyPhyGels in different configurations. (A) Schematic presentation of CyPhyGels used for the experiments in Figures 3, 4. (B) Fibroblasts grown on homogeneously soft CyPhyGels spread less than those grown on homogeneously stiff CyPhyGels. The same applies to separated CyPhyGels, while no difference was found among fibroblasts grown on heterogeneous CyPhyGels (Number of patients/number of cells). (C) Representative images of fibroblasts on different CyPhyGels. Green = cell membrane. Blue = nuclear counterstain. Scale bars = 20 μm (Number of patients/number of cells).