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. 2020 Jan 10;9:446. doi: 10.3389/fcimb.2019.00446

Figure 3.

Figure 3

Prolonged treatment of Ect cells with BV vaginal microbiota metabolite mixture at pH 7 elicits a dysfunctional inflammatory response. Ect cells seeded in a plate format were stimulated with a mixture of vaginal microbiota metabolites recapitulating BV (6 mM L-LA, 100 mM acetic acid, 20 mM succinic acid, 4 mM propionic acid and 4 mM butyric acid) for 24 h at pH 7 in the absence or presence of stimulation with the TLR agonists PIC and Pam and production of IL-6 (A), IL-8 (B), TNFα (C), IP-10 (D), RANTES (E), and MIP-3α (F) was quantified from supernatants. Graphs show mean and standard error of the mean from n = 4 independent assays. P ≤ 0.05 is denoted as * as determined by Mann-Whitney test. + denotes a significant difference (p < 0.05) as compared to untreated (UT) cells. BV, bacterial vaginosis; TLR, toll-like receptor; PIC, polyinosinic:polycytidylic acid; Pam, Pam3CSK4.