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. 2020 Jan 10;10:1657. doi: 10.3389/fpls.2019.01657

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Copyright © 2020 Park, Do, Kim, Park, Lee and Han

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Electrophoretic mobility shift assay of LcrX. EMSA was performed to determine between LcrX and putative promoter probes from (A) AOY60939 (fructose 1,6-bisphosphatase) and (B) AOY62452 (protease) labeled with [γ -³²P]. The binding reaction was performed using 0.5 μM of recombinant LcrX with 20 ng of labeled probes. Cold probes, 1X (20 ng), 10X (200 ng), and 50 X (1,000 ng), were added for a competition assay.