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. 2020 Jan 10;10:1657. doi: 10.3389/fpls.2019.01657

Figure 9.

Figure 9

Chrome azurol S (CAS) assay of Xag(EV), Xag(LcrX), XagΔlcrX(EV), and XagΔlcrX(LcrXP). Three microliters of a bacterial suspension at OD600 of 0.3 were dropped on (A and B) the iron-rich medium, XVM2-CAS, and (C and D) iron-limited medium, XVM2-CAS-BP which contained 2,2’-bipyrudyl (BP). (A and C) Photographs of colony and halos were taken (B and D), and their diameters were measured at 3 days after incubation. Black bars in the photographs represent 1 cm. Error bars indicate the means of five biological replicates with the standard deviation. Different letters on the error bar graph represent significant differences by one-way ANOVA (p < 0.05). This experiment was repeated at least three times.