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. 2020 Jan 16;10:439. doi: 10.1038/s41598-019-57360-4

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© The Author(s) 2020

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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The Syk inhibitor ameliorates EMT through suppression of the Syk/TGF-β1/Smad signaling pathway. (a) Western blot analysis of p-Syk/Syk, TGF-β1, Smad4, p-Smad2/Smad2 expression in HK-2 cells. (b–e) Statistical analysis of western blots for p-Syk/Syk, TGF-β1, Smad4, p-Smad2/Smad2 in HK-2 cells. Data are presented as means ± SD, n = 3 (three separate experiments). ^*P < 0.05, ^**P < 0.01, compared with control and ^#P < 0.05, ^##P < 0.01, ^###P < 0.001, compared with HG (Note: 80 µM FM; 1 µM BAY61-3606; HG,high glucose;FM,flavanomarein).