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. 2020 Jan 16;11:333. doi: 10.1038/s41467-019-14085-2

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Fig. 3 — a–d Soft agar colony formation assay with a, b overexpression of KDELR3 (KDELR3 OE) in human SK-MEL-28 cells vs. parental cell, unpaired two-tailed Student’s t test, P = 0.0015, d.f. = 10, t = 4.307. Six wells were analyzed per group. N = 6 (Control) and N = 6 (KDELR3 OE). c, d shRNA KDELR3 knockdown (KDELR3 KD) in human WM-46 cells vs. non-targeting control, unpaired two-tailed Student’s t test, P = 0.0324, d.f. = 10, t = 2.483. N = 6 wells analyzed per group. e, f Western blot and qPCR analysis of exogenously expressed FLAG-tagged KDELR3-001; ENST00000216014 (N) and KDELR3-001^Mu (Mu) in WM-46 (e) and 1205Lu (f) cells, transduced with non-targeting control (shControl/Cont./Control) or KDELR3-targeted (KD) shRNAs. Total KDELR3-001 RNA (KDELR3-001 and KDELR3-001^Mu) (f). g Rescue of soft agar colony formation in KDELR3-001^Mu cells (WM-46), Kruskal–Wallis with Dunn’s multiple comparison test. Five to six wells were analyzed per group. h, i Tail vein metastasis of Kdelr3 siRNA knockdown (Kdelr3 KD) in mouse B16 cells. Unpaired two-tailed Student’s t test with Welch’s correction, P = 0.0499, d.f. = 10.83, t = 2.207. N = 11 (siControl) and N = 11 (Kdelr3 KD). j Tail vein metastasis of KDELR3 siRNA-mediated knockdown in human 1205Lu cells transduced with Ferh-luc-GFP. Unpaired two-tailed Student’s t test with Welch’s correction, P = 0.0075, d.f. = 12.57, t = 3. N = 11 (siControl) and N = 11 (KDELR3 KD). b, d Bars and error bars depict mean ± s.e.m. g Bars and error bars depict mean ± SD. i, j Lines and error bars depict mean ± s.e.m. a–f, h–j Representative of three independent experiments. g Representative of two independent experiments. e β-tubulin loading control.