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. 2020 Jan 16;11:333. doi: 10.1038/s41467-019-14085-2

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© This is a U.S. government work and not under copyright protection in the U.S.; foreign copyright protection may apply 2020

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 4 — a Scatter plot of Eif2ak3 (Perk) RNA expression vs. Kdelr3 RNA expression. Linear regression analysis, R² = 0.3936, P < 0.0001. b Scatter plot of Ppp1r15a (Gadd34) RNA expression vs. Kdelr3 RNA expression. Linear regression analysis, R² = 0.1137, P = 0.03. a, b Data from four independent mouse models of melanoma (see Methods). Each dot represents one mouse. M1, N = 9 mice; M2, N = 6 mice; M3, N = 12 mice; M4, N = 13 mice. c Western blot analysis of PERK and eIF2α signaling (1205Lu cells). Non-targeting control (shControl, C) and KDELR3 knockdown (shKDELR3, KD) in cells were untreated or treated with 3 µg/ml tunicamycin (TM) or DMSO control for the indicated time. Immunoblot with antibodies specified, β-tubulin loading control. d Live/dead violet cell stain in KDELR3-knockdown 1205Lu cells. Untreated, DMSO, and tunicamycin (2.5 μg/ml) treatment groups were treated 18 h before collection. The right-hand peak on the graph indicates the percentage of dead cells. Representative of three independent experiments (c, d).