Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2020 Jan 16;11:332. doi: 10.1038/s41467-019-14029-w

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© The Author(s) 2020

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

PMC Copyright notice

Fig. 6 — a–d Single-cell RNA-seq analyses of lineage-traced Cxcl12-creER⁺ cells. a Cxcl12^CE-tdTomato⁺ cells were isolated from contralateral (CONT) and ablated (ABL) femurs separately at 7 days after marrow ablation. Two datasets were integrated by Seurat/CCA. b, c UMAP-based visualization of major classes of mesenchymal Cxcl12^CE-tdTomato⁺ cells (Cluster 0–11). Left panel, red dotted contour: reticular (Clusters 0–2). Lower panels: feature plots. Blue: high expression. Right panel: cells colored by conditions (CONT, ABL). Biological replicate 1, n = 2939 cells (CONT: 2066 cells, ABL: 873 cells), pooled from n = 7 mice. d Split-dot-based visualization of representative gene expression. Clusters 0 and 1 (reticular) and Cluster 6 (pre-osteoblast) shown for reticular-signature genes (Cxcl12, Adipoq) and osteoblast-signature genes (Col1a1, Bglap, Spp1, Alpl, Postn, Tnc, Pth1r, Sox9, Runx2, Sp7, Atf4). *p < 0.0001, Wilcoxon rank sum test. Circle size: percentage of cells expressing a given gene in a given cluster (0–100%), Color density: expression level of a given gene. e, f CFU-F assay of Cxcl12^CE-tdTomato⁺ bone marrow cells, harvested from contralateral (CONT, left) and ablated (ABL, right) femurs at Day 7 (pulsed at Day-7). Scale bars: 5 mm. g Percentage of tdTomato⁺ colonies among total CFU-Fs. n = 5 mice per group. *p < 0.05, two-tailed, Mann–Whitney’s U test. Data are presented as mean ± s.d. Source data are provided as a Source Data file.