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. 2020 Jan 16;11:331. doi: 10.1038/s41467-019-14044-x

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© The Author(s) 2020

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 2 — Heat-map visualization of a representative trapped ion mobility resolved mass spectrum of human plasma at an elution time of 9.2 min. Red dots indicate precursors selected for MS/MS fragmentation in the subsequent 100 ms PASEF scan in a, standard TIMS-MS/MS mode and b TIMS-PASEF mode. The dashed line indicates the positioning of the quadrupole. c Distribution of the number of precursors per PASEF scan in an LC-MS analysis of human plasma lipid extract (n = 1). d Total number of 4D features extracted from 30 min runs of human plasma (n = 5), mouse liver (n = 5), and human cancer cells (n = 5) in positive ion mode without (TIMS-MS/MS, red) and with PASEF (TIMS-PASEF, blue). The fraction of features with assigned MS/MS spectra is indicated by a darker color.