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. 2020 Jan 16;11(1):28. doi: 10.1038/s41419-019-2214-9

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© The Author(s) 2020

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 3 — a-c Liver sections from Acer3^+/+ and Acer3^−/− mice fed PEWD for 8 weeks were stained with TUNEL or a cleaved caspase 3 antibody a. Cells stained positive for TUNEL b and cleaved caspase 3 c were counted to evaluate hepatocellular apoptosis induced by NASH. d Liver tissues from the above mice were subjected to Western blot analyses with antibodies against cleaved caspase 3 or β-Actin (a protein loading control). Images in a represent results from five pairs of mice. ***P < 0.001.