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. 2020 Jan 16;11(1):34. doi: 10.1038/s41419-019-2188-7

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© The Author(s) 2020

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 7 — a qRT-PCR detected that CDK8 or LRP6 level in Hep G2 cells with DLGAP1-AS1 knockdown was upregulated by transfection of pcDNA3.1/CDK8 or pcDNA3.1/LRP6. b TOP/FOP flash assay was performed to verify the deactivating effect of DLGAP1-AS1 knockdown on TCF/LEF transcription, which was reactivated by CDK8 or LRP6 overexpression. c The influences of DLGAP1-AS1 knockdown and CDK8 or LRP6 overexpression on β-catenin expression, phosphorylation, and nuclear translocation were evaluated using WB analysis. d WB analysis displayed the protein levels of several typical downstream genes of Wnt/β-catenin pathway, which were downregulated by DLGAP1-AS1 knockdown and then upregulated by transfection of pcDNA3.1/CDK8 or pcDNA3.1/LRP6. e, f CCK-8 assay and wound healing assay showed that the inhibitory effects on cell proliferation and migration of DLGAP1-AS1 knockdown were attenuated by overexpression of CDK8 and LRP6, as well as by treatment with CHIR99021. g TUNEL assay showed that the promotional effect on cell apoptosis of DLGAP1-AS1 knockdown was attenuated by CDK8, LRP6 or CHIR99021. h, i The influences of CDK8 and LRP6 overexpression and CHIR99021 treatment on EMT-related factors in Hep G2 cells with DLGAP1-AS1 knockdown were respectively analyzed using qRT-PCR and WB. All data are presented as the mean ± SD of three independent experiments. *p < 0.05, **p < 0.01.