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. 2020 Jan 16;10:467. doi: 10.1038/s41598-019-57134-y

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© The Author(s) 2020

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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AVP receptor blockade partially reverts the decreased claudin-5 expression in the CNS of EAE rats. (a) Representative blot showing changes in the expression of the tight junction protein claudin‐5 in the brain and spinal cord of EAE group (EAE) and EAE conivaptan-treated group (CO) as compared with the intact controls (IC). (b) Graphs show the normalized optical density (OD) of protein expression for claudin‐5 in the IC, EAE and CO groups. A 220‐kDa band stained with Ponceau red was used for normalization of claudin-5 expression. Mean ± SD, *p ≤ 0.05 as compared with the intact control.