Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2020 Jan 16;11:330. doi: 10.1038/s41467-019-14055-8

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© The Author(s) 2020

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

PMC Copyright notice

Fig. 4 — a T-SNE plot displaying integrated E18.5 UT (blue) and AZT-treated (orange) oocytes colored by original identities. b T-SNE plot displaying cluster analysis of integrated E18.5 UT and AZT-treated oocytes. 11 clusters formed, cluster 11 removed from downstream analyses because top genes were mitochondrial, indicative of poor quality. Developmental trajectory based on known marker genes is indicated by arrows. c Heatmap of average expression of early (Ccnb3), middle (Dppa3), and late (Gdf9) marker genes within individual clusters. Clusters ordered from early to late developmental timepoints. d Percent of oocytes in untreated or AZT-treated samples belonging to each cluster, ordered from early to late developmental timepoints. Approximately 16,000 single-cells and 50,000 reads/cell collected from WT CD1 E18.5 untreated and AZT-treated ovaries from one litter of at least six pairs of ovaries each. Experiment repeated one time for both untreated and AZT-treated samples, but with ~5000 single-cells per sample and 7000–10,000 reads/cell (Supplementary Data 6c). e GM130 and L1 ORF1p expression in representative E18.5 and P2 Chk2^+/− and Chk2^−/− + AZT ovary sections. Scale bars: 5 μm. f Golgi area (μm²) in Chk2^+/− and Chk2^−/− untreated and AZT-treated E18.5 and P2 ovaries. Box plots indicate center line at median value, box limits at upper and lower quartile values, and whiskers at max and min values; n > 70 oocytes per sample. Stats by Kolmogorov–Smirnov test, ns p > 0.05; *p < 0.05; **p < 0.01; ***p < 0.001. g MVH and hematoxylin labeling of P4 and P19 Chk2^+/− and Chk2^−/− + AZT ovaries. Scale bars:100 μm. Inset exemplifies primordial (P) and non-primordial (NP) follicles. h Quantification of follicles in P4 and P19 ovaries. Dots indicate independent ovary samples; data are mean + s.e.m.; n > 3 ovaries per sample. i Number of litters from Chk2^+/−, Chk2⁻^/−, and Chk2^−/− + AZT females crossed to Chk2^+/− males. Dots indicate independent females; data are mean + SD; n > 3 females per condition. j Pups per litter from Chk2^+/−, Chk2⁻^/−, and Chk2^−/− + AZT females crossed to Chk2^+/− males. Dots indicate independent litters; data are mean + SD; n > 21 litters per condition. h–j Stats by two-tailed unpaired Student’s t-test, ns p > 0.05; *p < 0.01.