Comparison of the expression of apoptosis-related genes and proteins. Real-time PCR and Western blot detected the expression of apoptosis-related genes and proteins, including Bcl-2, Bax, Caspase-1, 3, and 8, and Cyt-c, after the Mcl-1-shRNA-treated mouse peritoneal macrophages were infected with H37Rv. A. Real-time PCR detected the relative expression of Bcl-2, Bax, Caspase-1, 3, and 8, and Cyt-c mRNA. B. Western blot detected the relative expression levels of Bcl-2, Bax, Caspase-1, 3, and 8, and Cyt-c protein. Data shown are the mean ± SD of three independent experiments.