Table 1.

Details of PCR and restriction enzyme digestion

Polymorphism	Primers	Annealing temperature (°C)	Restriction enzyme	Genotype and band sizes
MTHFR C677T	Forward: 5’-TGA AGG AGA ACG TGT CTG CGG GA-3’	59	HinfI	TT (175, 23 bp)
	Reverse: 5’-AGG ACG GTG CGG TGA GAG TG-3’			TC (198, 175, 23 bp)
				CC (198 bp)
MTHFR A1298C	Forward: 5’-CTT TGG GGA GCT GAA GGA CTA CTA C-3’	62	MboII	AA (56, 31, 30, 28, 18 bp)
	Reverse: 5’-CAC TTT GTG ACC ATT CCG GTT TG-3’			AC (84, 56, 31, 30, 18 bp)
				CC (84, 31, 30, 18 bp)
NFKB1 -94ins/del ATTG	Forward: 5’-TGG GCA CAA GTC GTT TAT GA-3’	60	Van91I	II (240, 45 bp)
	Reverse: 5’-CTG GAG CCG GTA GGG AAG-3’			ID (281, 240, 45 bp)
				DD (281 bp)
NFKBIA 3’UTR A>G	Forward: 5’-GGC TGA AAG AAC ATG GAC-3’	58	HaeIII	AA (424 bp)
	Reverse: 5’-GTA CAC CAT TTA CAG GGA GGG-3’			AG (424, 316, 108 bp)
				GG (316, 108 bp)
DAZL A386G (T54A)	Forward: 5’-GAA TGC TGA ATT TTT ACT CTT GAA G-3’	62	AluI	TT (115, 66 bp)
	Reverse: 5’-CTC TAT ACG TGG CTA GAG TTC-3’			TA (115, 66, 53 and 13 bp)
				AA (115, 53 and 13 bp)
CYP1A1 T3801C	Forward: 5’-TAG GAG TCT TGT CTC ATG CCT-3’	58	MspI	TT (340 bp)
	Reverse: 5’-AGC GGC TAC ACC TCT TCA CTG 3’			TC (340, 200, 140 bp)
				CC (200, 140 bp)