Figure 1.

Capn4 promotes proliferation, migration, and invasion of RCC cells. A. Stable up- or down-regulation of Capn4 expression in transfected RCC cell lines was confirmed by qRT-PCR. B, C. In the CCK8 assay, cell proliferation was significantly increased after Capn4 over-expression (P<0.01) and significantly decreased after Capn4 shRNA transfection (P<0.05). D. Wound-healing migration assays and the quantification of the percentage of open area are shown. E. The invasion of cancer cells was measured by transwell Matrigel invasion assays; data are shown are mean ± SEM from three independent experiments. Statistical significance was assessed by Student’s t-test; *P<0.05, **P<0.01, ***P<0.001; scale bar =100 μm.