Figure 4.

NF-κB activation is critical to the Capn4 enhanced RCC cells proliferation. A. Western blot analysis revealed that the up-regulated phosphorylation levels of FAK and NF-κB in the Caki1-Capn4 group, while only the phosphorylation of NF-κB is completely abolished with QNZ treatment (3 μM for 24 h) compared to the respective control cells. B. Western blot analysis revealed that the down-regulated phosphorylation levels of FAK and NF-κB in the 780-O-shCapn4 group, while the phosphorylation level of NF-κB is further decreased with QNZ treatment (3 μM for 24 h) compared to the respective control cells. C. Inhibition of NF-κB phosphorylation using a QNZ (3 μM for 24 h) in Caki1 cells abolished the increase in cancer cell growth induced by Capn4 over-expression, and NF-κB inhibitor alone or combined with Capn4 shRNA led to a similar decrease in 780-O cell growth with Capn4 shRNA alone; data are shown are mean ± SEM from three independent experiments. Statistical significance was assessed by Student’s t-test; P<0.05, **P<0.01, ***P<0.001.