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FISH results (CEP17 stained green and HER-2 gene stained red). A. Negative, a ration of HER-2 to CEP17 signals less than 2.0 and with an average HER2 copy number < 4.0 signals/cell. B. Equivocal: a ratio of HER-2 to CEP17 signals < 2.0 with an average HER2 copy number ≥ 4.0 and < 6.0 signals/cell. C. HER-2 gene amplification: red signal were clustered obvious. D. HER-2 gene amplification: a ratio of HER-2 to CEP17 signals < 2.0 but the average HER2 copy number ≥ 6.0 signals/cell.
