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. 2017 Jul 1;10(7):8072–8081.

Figure 4.

Figure 4

A. Immunofluorescence staining of olfactory receptor neuron specific markers (OMP, Olfactory Maker Protein). Aa. The iPS cells co-cultured with OSNs hardly expressed Oct4 GFP Gene. Ab. The differentiated iPSCs expressed OMP after 12-day co-culture Scale bars = 50 um. Ac. 4’,6-diamidino-2-phenylindole (DAPI) staining. Ad. Image D is merged by image B and image C. Scale bars = 50 um. B. Immunofluorescence staining of olfactory receptor neurons specific Markers (NCAM, Neural Cell Adhesion Molecule). Ba. The iPS cells co-cultured with OSNs hardly expressed Oct4 GFP Gene. Bb. The differentiated iPSCs expressed NCAM after 12-day co-culture. Bc. 4’,6-diamidino-2-phenylindole (DAPI) staining. Bd. Image D is merged by image B and image C. Scale bars = 50 um. C. Immunofluorescence staining of olfactory receptor neurons specific Markers (GAP43, Growth Associated Protein-43). Ca. The iPS cells co-cultured with OSNs hardly expressed Oct4 GFP Gene. Cb. The differentiated iPSCs expressed GAP43 after 12-day co-culture. Cc. 4’,6-diamidino-2-phenylindole (DAPI) staining. Cd. Image D is merged by image B and image C. Scale bars = 50 um.