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. 2017 Jul 1;10(7):8072–8081.

Figure 5.

Figure 5

A. Immunofluorescence staining of mitral/tufted neurons specific markers (TBX21, T-box Transcription Factor-21). Aa. The iPS cells co-cultured with OSNs hardly expressed Oct4 GFP Gene. Ab. The differentiated iPSCs expressed TBX21 after 12-day co-culture. Ac. 4’,6-diamidino-2-phenylindole (DAPI) staining. Ad. Image D is merged by image B and image C. Scale bars = 50 um. B. Immunofluorescence staining of mitral/tufted neurons specific markers (Iba1, Ionized Calcium Binding Adaptor Molecule 1). Ba. The iPS cells co-cultured with OSNs hardly expressed Oct4 GFP Gene. Bb. The differentiated iPSCs expressed Iba1 after 12-day co-culture. Bc. 4’,6-diamidino-2-phenylindole (DAPI) staining. Bd. Image D is merged by image B and image C. Scale bars = 50 um.