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. 2020 Jan 16;10:474. doi: 10.1038/s41598-019-57173-5

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© The Author(s) 2020

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Long-term storage of heparinized blood causes elevation of in-circuit pressure. (A) A schema of the extracorporeal recirculation circuit is shown. The circuit consists of polyvinyl chloride (PVC) tubes, a pooling reservoir, a roller pump, a manometer, and a removable filter with a pore size of 40 µm. (B) Heparinized blood stored for 0, 1, 3, or 5 days was administered to the circuit and in-circuit pressure was monitored for up to 60 minutes. N = 5 per group. (C) Citrated blood stored for 0, 1, 3, or 5 days was administered into the circuit and in-circuit pressure was monitored for up to 60 minutes. N = 5 per group. Repeated measures analysis of variance models were used to analyze changes in the area under the curve (AUC) over time. *p < 0.05, **p < 0.01.