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. 2020 Jan 16;10:474. doi: 10.1038/s41598-019-57173-5

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© The Author(s) 2020

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Extracellular DNA contributes to elevation of in-circuit pressure. (A) Citrated whole blood stored for 7 days was administered to the circuit and then 5 U/mL of unfractionated heparin was administered at 15 minutes. Deoxyribonuclease (DNase), ribonuclease (RNase), or proteinase was administered at 30 minutes and in-circuit pressure was monitored for up to 60 minutes. N = 4 per group. A waveform of in-circuit pressure in RNase group did not represent because it was almost same as that of no treatment. (B) The paired t-test was used to analyze the difference in the area under the curve (AUC) between DNase treatment and no treatment. *p < 0.05. (C) The filter was removed at 60 minutes and analyzed by immunofluorescence. Fibrin(ogen) was labeled in red and DNA was labeled in blue. Representative images of the whole filter (scale bars: 10 mm) and the magnified view (scale bars: 50 μm) are shown.