Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2020 Jan 16;10:474. doi: 10.1038/s41598-019-57173-5

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© The Author(s) 2020

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

PMC Copyright notice

Leukocyte stimulation leads to extracellular DNA deposition and elevation of in-circuit pressure. (A) Citrated whole blood on day 0 was stimulated with 0.6 µg/ml phorbol 12-miristate 13-acetate (PMA) at 37 °C for 120 minutes before administration to the circuit. Unfractionated heparin (Hep) was administered at 15 minutes and in-circuit pressure was monitored for up to 60 minutes. N = 4 per group. (B) The paired t-test was used to analyze the difference in the area under the curve (AUC) between the PMA-stimulated group and the non-stimulated group. *p < 0.05. (D) The filter was removed at 60 minutes and analyzed by immunofluorescence. Fibrin(ogen) was labeled in red and DNA was labeled in blue. Representative images of the whole filter (scale bars: 10 mm) and the magnified view (scale bars: 50 μm) are shown.