Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2020 Jan 10;10:3046. doi: 10.3389/fimmu.2019.03046

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

Copyright © 2020 Tay, Kanellakis, Hosseini, Cao, Toh, Bobik and Kyaw.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

PMC Copyright notice

Transfer of wildtype, not MHCII- or CD40-knockout B2 cells promote atherosclerosis in μMT^−/− ApoE^−/− mice. Spleen B2 B cells isolated and purified from different donors (see text for detail) were adoptively transferred into μMT^−/− ApoE^−/− mice at the beginning of 8 week HFD. At the end of experiment, FACS analysis showed that (A) transferred B cells were detected in recipient spleens. Atherosclerosis at aortic sinus was assessed by (B) total intimal lesion areas in ORO-stained aortic sinus sections. Wildtype, not MHCII- or CD40-deficient B2 B cells augmented atherosclerosis without affecting (C) lipid and (D) macrophage accumulation expressed as per lesion area. Lesion immune cell analysis showed that (E–F) CD4 T and CD8 T cell accumulation was reduced in μMT^−/− ApoE^−/− mice. Data presented as mean ± SEM of two independent experiments. n = 9 or more per group, *p < 0.05 compared to PBS transferred group. □ PBS transfer, ■ WT B cell transfer, MHCII^−/− B cell transfer, and CD40^−/− B cell transfer.

Inline graphic — Transfer of wildtype, not MHCII- or CD40-knockout B2 cells promote atherosclerosis in μMT^−/− ApoE^−/− mice. Spleen B2 B cells isolated and purified from different donors (see text for detail) were adoptively transferred into μMT^−/− ApoE^−/− mice at the beginning of 8 week HFD. At the end of experiment, FACS analysis showed that (A) transferred B cells were detected in recipient spleens. Atherosclerosis at aortic sinus was assessed by (B) total intimal lesion areas in ORO-stained aortic sinus sections. Wildtype, not MHCII- or CD40-deficient B2 B cells augmented atherosclerosis without affecting (C) lipid and (D) macrophage accumulation expressed as per lesion area. Lesion immune cell analysis showed that (E–F) CD4 T and CD8 T cell accumulation was reduced in μMT^−/− ApoE^−/− mice. Data presented as mean ± SEM of two independent experiments. n = 9 or more per group, *p < 0.05 compared to PBS transferred group. □ PBS transfer, ■ WT B cell transfer, MHCII^−/− B cell transfer, and CD40^−/− B cell transfer.