Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2020 Jan 10;10:3046. doi: 10.3389/fimmu.2019.03046

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

Copyright © 2020 Tay, Kanellakis, Hosseini, Cao, Toh, Bobik and Kyaw.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

PMC Copyright notice

Wildtype and CD40-deficient B2 cells restore plasma immunoglobulins, however only wildtype B2 transfer enhances CD4 T cell activation and their production of TNF-α and IFN-γ in μMT^−/− ApoE^−/− mice. B2 B cells were adoptively transferred into μMT^−/− ApoE^−/− mice at the beginning of 8 week HFD. At the end of experiment, plasma immunoglobulin levels determined by ELISA showed that wild-type and CD40^−/− B2 B cell transfer groups increased (A) total IgG, total IgM and (B) oxLDL-IgG and oxLDL-IgM subclasses. However, only wildtype B2 cells increased (C) total CD4 T cells, (D) activated CD44⁺ CD4 T cells and (E) TNF-α- and (F) IFN-γ-producing CD4 T cells in spleens. Data presented as mean ± SEM of two to three independent experiments. n = 9 per group, *p < 0.05 compared to PBS transferred group. □ PBS transfer, ■ WT B cell transfer, MHCII^−/− B cell transfer, and CD40^−/− B cell transfer.

Inline graphic — Wildtype and CD40-deficient B2 cells restore plasma immunoglobulins, however only wildtype B2 transfer enhances CD4 T cell activation and their production of TNF-α and IFN-γ in μMT^−/− ApoE^−/− mice. B2 B cells were adoptively transferred into μMT^−/− ApoE^−/− mice at the beginning of 8 week HFD. At the end of experiment, plasma immunoglobulin levels determined by ELISA showed that wild-type and CD40^−/− B2 B cell transfer groups increased (A) total IgG, total IgM and (B) oxLDL-IgG and oxLDL-IgM subclasses. However, only wildtype B2 cells increased (C) total CD4 T cells, (D) activated CD44⁺ CD4 T cells and (E) TNF-α- and (F) IFN-γ-producing CD4 T cells in spleens. Data presented as mean ± SEM of two to three independent experiments. n = 9 per group, *p < 0.05 compared to PBS transferred group. □ PBS transfer, ■ WT B cell transfer, MHCII^−/− B cell transfer, and CD40^−/− B cell transfer.