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. 2020 Jan 10;10:1536. doi: 10.3389/fphar.2019.01536

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Copyright © 2020 Zhu, Lu, Jiang, Yao, Jiang, Huang, Jiang, Peng, Fu and Zhao

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Islet viability and transmission electron microscopy (TEM) results in vitro. (A) Islet viability at 0 h. (B) Islet viability of each group after cultured for 7 h. (C) Islet viability of each group after culturing for 24 h. (D) Viability statistics after cultivation. The ratio of green to green plus red cells provided the percentage of viability. The three columns of each group represent 0, 7, and 24 h from left to right (****P < 0.0001, ***P < 0.001, 0 vs 24 h; ^#### P < 0.0001, 0 vs 7 h). (E) TEM of each group after 24 h culturing.a and A: FREE group; b and B: 60 ng bFGF group; c and C: 1 mg RHC group; d and D: 16 mg RHC group; e and E: 1 mg–60 ng RHC-bFGF group; f and F: 16 mg–60 ng RHC-bFGF group; N: nucleus; M: mitochondria; *: secretory granules.