TABLE 1.
Overview of the putative tunicaminyluracil gene clusters (TGC) evaluated in the current study.
| Species | No. of sequences analyzed | No. of TGC genesa | TGC size (bp)b | GC content of TGC (%) | GC content of genome (%) | MIC (μg/mL)c | References |
| Rathayibacter toxicus | 26 | 14 | 13,402 | 52 | 61.5 | 8.0 | Arif et al., 2016; Sechler et al., 2017; Davis et al., 2018 |
| Rathayibacter iranicus | 6 | 16 | 14,104 | 56 | 67.1 | 8.0 | This study and Davis et al., 2018 |
| Rathayibacter agropyri | 12 | 14 | 14,067 | 56 | 68.0 | Variabled | This study and Davis et al., 2018 |
| Rathayibacter woodii | 1 | 14 | 16,384 | 57 | 65.4 | 8.0 | This study |
| Rathayibacter rathayi | 1 | – | – | – | 69.3 | <0.0625 | Davis et al., 2018 |
| Rathayibacter tritici | 2 | – | – | – | 69.5 | <0.0625 | NZ_CP015515; PRJNA429011 |
| Streptomyces chartreusis | 1 | 14 | 11,988 | 65 | 70.8 | nd | Doroghazi et al., 2011 |
| Streptomyces clavuligerus | 1 | 14 | 12,056 | 67 | 72.3 | nd | Song et al., 2010 |
| Actinosynnema mirum | 1 | 12 | 10,993 | 75 | 73.7 | nd | Land et al., 2009 |
aThe number of putative genes present in the identified tunicaminyluracil gene clusters. bThe TGC size is based on the reference strains, R. toxicus FH79 (Sechler et al., 2017), R. iranicus FH6, R. agropyri CA4 (Schroeder et al., 2018), R. woodii FH236, S. chartreusis NRRL 3882 (Doroghazi et al., 2011), S. clavuligerus NRRL 3585 (Song et al., 2010), and A. mirum DSM 43827 (Land et al., 2009). cThe tunicamycin-minimum inhibitory concentration (MIC) values for evaluated Rathayibacter species. dSee Figure 3 for details. −, Not applicable; nd, not determined.