TABLE 2.
Genes associated with the putative tunicaminyluracil-related gene clusters of Rathayibacter toxicus FH79 (Sechler et al., 2017), R. agropyri CA4 (Schroeder et al., 2018), R. iranicus FH164, R. woodii FH236, Streptomyces chartreusis 3882 (Doroghazi et al., 2011), and Actinosynnema mirum DSM 43827 (Land et al., 2009).
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Protein length (aa) and% amino acid identity relative to R. toxicus FH79b |
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| Genea | Description | R. toxicus | R. agropyri | R. iranicus | R. woodii | S. chartreusis | A. mirum |
| tunA | Epimerase | 330 | 320(54%) | 320(53%) | 313(46%) | 321(42%) | 322(38%) |
| tunB | Radical SAM | 337 | 337(86%) | 337(84%) | 338(71%) | 338(68%) | 340(67%) |
| tunC | Acetyltransferase | 333 | 331(52%) | 333(51%) | 330(38%) | 318(28%) | 318(29%) |
| tunD | Glycosyltransferase | 454 | 453(55%) | 453(55%) | 456(40%) | 472(37%) | 451(38%) |
| tunE | N-deacetylase | 232 | 230(62%) | 230(62%) | 222(49%) | 234(42%) | 230(43%) |
| tunF | Epimerase | 320 | 312(60%) | 312(60%) | 338(45%) | 327(35%) | 328(35%) |
| tunG | Histidine phosphatase | 192 | − | − | 192(36%) | 203(31%) | 223(30%) |
| tunH | Nucleotide pyrophosphatase | 515 | 509(50%) | 509(48%) | 499(39%) | 515(38%) | 510(37%) |
| tunI | ABC transporter | 330 | 320(49%) | 320(50%) | 324(34%) | 304(32%) | 302(29%) |
| tunJ | ABC-2 transporter | 262 | 261(65%) | 261(64%) | 260(47%) | 262(44%) | 253(47%) |
| tunK | Acyl carrier protein | 96 | 96(45%) | 96(44%) | 98(33%) | 81(30%) | 85(28%) |
| tunL | Phospholipid phosphatase | 247 | 239(32%) | 243(31%) | 537(22%) | 229(22%) | 293(23%) |
| tunM | Methyltransferase | − | − | − | − | 216 | − |
| tunN | Pyrophosphatase | − | − | − | − | 152 | − |
| tunO | Hypothetical protein | 224 | − | − | − | − | − |
| tunP | Polyketide synthase | 407 | 408(57%) | 407(58%) | 408(48%) | − | − |
| tunQ | Enoyl-CoA hydratase/isomerase | − | 351 | 351 | 279 | − | − |
| tunR | 4′-phosphopantetheinyl transferase | − | 224 | 224 | − | − | − |
| tunS | Hypothetical protein | − | − | 122 | − | − | − |
| tunT | Hypothetical protein | − | − | 111 | − | − | − |
aGenes in bold text are essential for tunicamycin biosynthesis and immunity in Streptomyces chartreusis (Widdick et al., 2018). b–, Absence of gene.