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2D-clinostat and establishment of simulated microgravity system in HUVECs. a 2D-clinostat consists of cap, racks, coverslips and chamber. b The chamber without medium. c The chamber filled with 10% FBS, high-glucose DMEM medium. d The chambers of Con groups rotate around the vertical axis to act as rotation controls. e The chambers of MG groups rotate around the horizontal axis to avoid mechanical gravity environment. The speed of 2D-clinostat is 30 rpm
