Fig. 11.

The overexpression of ZHX1 diminishes the effect of protecting HUVECs from apoptosis induced by increase of miR-27b-5p. a–d Western blot analysis of Bcl-2, Bax and Cleaved Caspase-3 protein expression levels in HUVECs after co-transfection with miR-27b-5p mimics and pcDNA3.1-ZHX1 or their NC for 48 h. e Images of HUVECs stained with Hoechst 33342 after co-transfection with miR-27b-5p mimics and pcDNA3.1-ZHX1 or their NC for 48 h. Scale bar, 50 μm. Data are presented as mean ± SD. N = 3 in each group, *P < 0.05 versus 27-mimics NC or 27-mimics + pcDNA3.1-NC