Fig. 6.

Effect of miR-27b-5p over-expression on apoptosis in HUVECs under simulated microgravity. a–d Western blot analysis of apoptosis-related protein expression levels in HUVECs after transfection with 27-mimics or its NC in a simulated microgravity environment for 48 h. e Images of HUVECs stained with Hoechst 33342 after transfection with 27-mimics or its NC in a simulated microgravity environment for 48 h. Scale bar, 50 μm. Data are presented as mean ± SD. N = 3 in each group, *P < 0.05 versus MG + 27-mimics NC