Fig. 8.

Validation of ZHX1 functioning as the target gene of miR-27b-5p in HUVECs. a A schematic diagram of luciferase reporters composed of pmiR-WT-ZHX1-3′UTR REPORT or pmiR-MUT-ZHX1-3′UTR REPORT. b The effects of miR-27b-5p mimics or its NC on the luciferase activity of pmiR-WT-ZHX1-3′UTR or pmiR-MUT-ZHX1-3′UTR REPORT in HUVECs. c, d qRT-PCR analysis of ZHX1 mRNA expression level in HUVECs after transfection with 27-mimics, 27-inhibitor or their NC for 48 h. e, f Western blot analysis of ZHX1 protein expression level in HUVECs after transfection with 27-mimics, 27-inhibitor or their NC for 48 h. Data are presented as mean ± SD. N = 3 in each group, *P < 0.05 versus 27-mimics NC or 27-inhibitor NC