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. 2020 Jan 10;10:2988. doi: 10.3389/fmicb.2019.02988

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Copyright © 2020 Zhang, Zhang, Dai, Liang, Zhu, Pan, Zhang, Yan, Zhu, Zhang, Dai, Cao, Gu, Xue, Cao, Hu and Gong.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Depletion of BmMETTL3, BmMETTL14, and BmYTHDF3 expression levels with siRNAs and its effect on replication of BmNPV. (A) Viability assay of siRNA-treated cells. (B) The expression levels of BmMETTL3, BmMETTL14, and BmYTHDF3 gene in the siRNA-treated cells were detected with real-time PCR. (C) Effect of depletion of BmMETTL3, BmMETTL14, and BmYTHDF3 genes on VP39 expression. Statistically significant differences between the mean values were determined by Student’s t-test (^∗p < 0.05, ^∗∗p < 0.01, and ^∗∗∗p < 0.001).