Figure 3.

RKIP overexpression inhibited miR-200a induced increases of cell viability and invasion in human esophageal squamous cell carcinoma TE3 cells via inactivating MAP/ERK pathway. Cells were transfected with RKIP overexpression vector (RKIP) and/or miR-200a mimic. MTT and Transwells assays was performed to determine cell viability at corresponding time (A) and invasiveness (B). Protein expressions of RKIP, Raf1, phosphorylated (p)-Raf1, ERK-1/2 and p-ERK1/2 were measured by western blotting (C). Statistical results of phosphorylation levels about Raf1 and ERK1/2 to assess the activation of MAPK/ERK pathway in TE3 cells (D). *, P < 0.05; **, P < 0.01; compared to that in corresponding time point of control group. ***, P < 0.001 compared to the corresponding negative control.