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. 2017 Aug 1;10(8):8928–8935.

Figure 2.

Figure 2

A. Immunofluorescence analysis of Bmi-1 expression in A431 cell line. Bmi-1 was dominantly expressed in the cytoplasm and nucleus of the A431 cell line. B. Bmi-1 expression was detected by Western blot after transfected for 48 h with Bmi-1-shRNA in A431 cells, while shRNA#1 achieved the more effected down-regulation. The relative level of Bmi-1 was tested by densitometry. The date are mean ± SEM *P<0.05 compared with the control. C. Fluorescence observation of the transfection efficiency shRNA-GFP in A431 cell line (magnification ×100).