Figure 2.

Overexpression of SFRP1 suppresses cell proliferation and tumorigenesis. A. MTT assay was performed to assess the viability of both Hep-2 and SNU899 cells transfected with SFRP1-expressing plasmid or vector after culturing for 48 or 72 h. B. Colony formation assay. Hep-2 and SNU899 cells stably expressing SFRP1 or vector were seeded at a low density and cultured for 10 days to allow to form colonies. C. Tumorigenic studies in nude mice. Hep-2 cells stably expressing SFRP1 and control cells were inoculated into nude mice, and tumor development was monitored for 5 weeks. Reexpression of SFRP1 significantly inhibited the growth of Hep-2 xenograft tumors. D. Tumor sections were subjected to Ki-67 immunohistochemistry. Representative images of Ki-67 staining are shown top panels. Scale bar = 50 μm. *P < 0.05 vs. the vector group.