Figure 5.

Overexpression of NHE1 rescues the inhibitory effects of SFRP1 on Hep-2 cells. A. Western blot analysis of NHE1 protein levels in Hep-2 cells transfected with the SFRP1-expressing plasmid or together with the NHE1-expressing plasmid. Numbers below the Western blots indicate fold-change in NHE1 protein levels. B. Measurement of intracellular pH values using the pH-sensitive probe BCECF-AM. C. Hep-2 cells transfected with indicated constructs were assessed for proliferation after culturing for 48 or 72 h. D. Analysis of apoptosis in Hep-2 cells transfected with indicated constructs by flow cytometry after annexin-V/PI staining. *P < 0.05.