Fig. 6.

Effect of cytochalasin B on acute heat shock of camel cells. (A) cells were incubated at 38 °C and with 0.5 mg/mL cytochalasin B (CB). (B and C) Cell were treated with or without CB at 45℃, respectively. (D, E, and F) Images of A, B, and C, at the end of heat treatment for two hours. CB at 38 °C caused cell shrinkage (D. arrows), the effect was exaggerated at 45 °C (E. arrows). (F) shows no morphological changes of cells exposed to 45 °C without addition of CB. (G, H, and I) show the cells two hours post-washing (PW) and medium replacement without CB in all treatments. Cells were microscopically examined for identifying the effects of CB. (H) Cells exposed to 45 °C in the presence of CB show cell degeneration and disturbed architecture (arrows). Scale bar = 100 µm.