The effect of supplementing ROCK-inhibitor Y-27632 (RI) during acute heat shock on wound healing assay. (A–C) Cell monolayers 0 h after wound healing. (D–F) Cells after 24 h at 38 °C. (G–I) Cells after 48 h at 38 °C. (A, D, and G) RI-treated cells for 2 h at 38 °C. (B, E, and H) RI-treated cells for 2 h at 45 °C. (C, F, and I) non-treated cells cultured for 2 h at 45 °C. (J) results of wound healing and wound closure in cells of the three treatments in µm. Scale bar = 500 µm. Lower case letters (a, b, and c) indicate significant differences at P < 0.05.