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. 2019 Nov 30;19:731–740. doi: 10.1016/j.omtn.2019.10.048

Figure 1.

Figure 1

Increased Expression of FBW7 in Colon Tissues of Inflammatory Bowel Disease (IBD) Patients and the Trinitrobenzene Sulphonic Acid (TNBS)-Induced Mouse Colitis Model

(A) The mRNA expression of FBW7 in colon tissues from control individuals (non-IBD, n = 189) and patients with Crohn’s disease (CD; n = 172) and patients with ulcerative colitis (UC; n = 147) was determined by RT-PCR. **p < 0.01 versus non-IBD. (B) Western blotting analysis of FBW7 protein expression in non-IBD individuals and patients with CD and patients with UC; n = 40 in each group. **p < 0.01 versus non-IBD. (C) Representative images of hematoxylin and eosin staining and immunohistochemistry for FBW7 expression. Scale bar, 50 μm. (D) The mRNA expression of FBW7 in blood from IBD patients was determined; n = 40 in each group. (E and F) A mouse acute experimental colitis was induced by rectal injection with TNBS. The expression of FBW7 in colon tissues was analyzed by RT-PCR (E) and western blotting (F). **p < 0.01 versus control, n = 12. (G) Representative images of immunohistochemistry for colonic FBW7 expression; n = 4 in each group. Scale bar, 50 μm.