Figure 3.

miR-129 Directly Targets FBW7 3′ UTR

(A) Predicted miR-129 seed matches the sequence in the 3′ UTR of FBW7 in human and mouse species. (B and C) Caco-2 cells were transfected with miR-129 mimics (MiR-129-m; 10 nM) or mimics negative control (NC-m) (B) or miR-129 inhibitor (MiR-129-I; 10 nM) or inhibitor negative control (NC-i) (C) for 48 h. The expression of miR-129 was determined by RT-PCR. (D and E) Dual luciferase activity assay was performed by cotransfection of luciferase reporter containing FBW7 3′ UTR or the mutant one with miR-129 mimics (D) or miR-129 inhibitor (E) in Caco-2 cells. (F and G) Western blotting analysis of FBW7 protein expression in cells transfected with miR-129 mimics (F) and miR-129 inhibitor (G). All data were presented as mean value ± SEM. **p < 0.01 versus control; n = 6.