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. 2019 Nov 29;19:643–653. doi: 10.1016/j.omtn.2019.10.047

Figure 3.

Figure 3

UCA1 Affect the Proliferation and Apoptosis of NSCLC In Vitro

(A) Quantitative real-time PCR analysis of UCA1 in HCC827GR and PC9GR cells transfected with UCA1 siRNAs for 48 h. (B) Quantitative real-time PCR analysis of UCA1 in HCC827 and PC9 cells transfected with pcDNA-Control or pcDNA-UCA1 for 48 h. (C) CCK-8 assay showing UCA1 silencing suppressed the proliferation of HCC827GR cells in the presence of 1 μM gefitinib. (D) CCK-8 assay showing UCA1 silencing suppressed the proliferation of PC9GR cells in the presence of 1 μM gefitinib. (E) CCK-8 assay showing overexpression of UCA1 promoted the proliferation of HCC827 cells in the presence of 1 μM gefitinib. (F) CCK-8 assay showing overexpression of UCA1 promoted the proliferation of PC9 cells in the presence of 1 μM gefitinib. (G) Flow cytometry analysis of apoptosis in PC9GR and HCC827GR cells transfected with si-Control or si-UCA1 prior to the stimulation of 0.1 μM gefitinib treatment for 36 h. (H) Flow cytometry analysis of apoptosis in PC9 and HCC827 cells transfected with pcDNA-Control or pcDNA-UCA1 prior to the stimulation of 0.1 μM gefitinib treatment for 36 h. All tests were performed at least three times. Data were expressed as mean ± SD. **p < 0.01.