Figure 5.

UCA1 Functions as a Molecular Sponge of miR-143 in NSCLC Cells

(A) starBase v.2.0 results showing the sequence of UCA1 with highly conserved putative miR-143 binding sites. (B) miR-143 mimic considerably reduced the luciferase activity of the WT-UCA1 luciferase reporter vector compared with negative control, whereas miR-143 mimic did not pose any impact on the luciferase activity of MUT-UCA1-transfected cells. (C) UCA1 and miR-143 simultaneously existed in the production precipitated by anti-AGO2. (D) Relative expression of miR-143 in the gefitinib-sensitive group and gefitinib resistance group in NSCLC patients. (E) Relative expression of miR-143 in a panel of NSCLC cell lines. All tests were performed at least three times. Data were expressed as mean ± SD. **p < 0.01.