Figure 7.

FOSL2 Was a Direct Target of miR-143

(A) Bioinformatics analysis revealed the predicted binding sites between FOSL2 and miR-143. (B) Luciferase reporter assay demonstrated miR-143 mimics significantly decreased the luciferase activity of FOSL2-WT in NSCLC cells. (C) IHC analysis of FOSL2 in the gefitinib-sensitive group in NSCLC patients and the gefitinib resistance group. (D) Relative expression of FOSL2 in a panel of NSCLC cell lines. (E) Overexpression of miR-143 significantly decreased the protein expression of FOSL2 in PC9 cells. (F) The effect of knockdown UCA1 on mRNA levels of FOSL2 was attenuated by miR-143 inhibitor. (G) The effect of knockdown UCA1 on protein levels of FOSL2 was attenuated by miR-143 inhibitor. All tests were performed at least three times. Data were expressed as mean ± SD. **p < 0.01.