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. 2019 Dec 24;19:627–642. doi: 10.1016/j.omtn.2019.12.018

Figure 2.

Figure 2

Inhibitory Potential of siRNAs against A/California/04/2009 (H1N1) in MDCK Cells

(A) Quantitative analysis of viral RNA by real-time PCR. RNA copy number of siRNA-treated samples at 8 nM concentration was compared to the RNA copy number of negative control K1 (established as 100%). (B) Graph of viral RNA copy numbers by real-time PCR for the most effective siRNAs tested at three concentrations. Cells treated with Lipofectamine 2000 without any siRNA are marked as LF; INF stands for untreated and infected cells. The error bars represent the standard deviations from three independent experiments. The unpaired two-tailed Student’s t test was performed for statistical comparisons (*p < 0.05, **p < 0.01, ***p < 0.001).