Figure 6.

Inhibitory Potential of Modified siRNAs at 8 nM Concentration against A/California/04/2009 (H1N1) in MDCK Cells

(A and B) Quantitative analysis of viral RNA copy number performed by real-time PCR for (A) siRNAs containing 2′-fluororibonucleotides and (B) siRNAs modified with thiophosphates, 2′-O-methyl-siRNAs, and siDNA. Cells treated with Lipofectamine 2000 without any siRNA are marked as LF. The error bars represent the standard deviations from three independent experiments. The unpaired two-tailed Student’s t test was performed for statistical comparisons (*p < 0.05, **p < 0.01, ***p < 0.001).