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. 2020 Jan 16;10:512. doi: 10.1038/s41598-020-57545-2

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© The Author(s) 2020

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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In vivo rescue of rZIKV by inoculation of different concentrations of pBAC-ZIKV/LPF. Four-to-five-week-old IFNAR−/− A129 male (N = 3) and female (N = 3) mice were inoculated IP with 100, 50, 25, 12.5 or 6.25 µg (top to bottom) of pBAC-ZIKV complexed with LPF as described in Fig. 2. Body weight loss (A) and survival (B) were evaluated at the indicated dpi. Mice that lost more than 20% of their initial body weight or presented hind limb paralysis were humanely euthanized. Error bars represent SD of the mean for each group of mice. Mice were bled at 3, 6 and 9 dpi and viral titers in sera (C) were determined by immunofocus assay (FFU/ml). Symbols represent data from individual mice and bars the geometric means of viral titers. *Virus not detected in one mouse; &, virus not detected in two mice; ND, not detected. Dotted black lines indicate the LoD (200 FFU/ml). Statistically significant differences (p = 0.008) in survival were observed using a Long-rank test. No statistically significant differences (p = 0.179) in viral titers were observed using ANOVA test.