Figure 5.

Insulin‐induced Na⁺ transport and SGK1 activity. (a) Confluent cells on Costar Snapwell membranes were mounted in Ussing chambers so that the effects of insulin (20 nM) upon I _Amil could be continuously recorded (mean ± 95% CI, n = 6) under control conditions (left panel) and in cells that had been pretreated (2 hr) with 1‐μM TSA (middle panel). The amiloride‐sensitive responses to insulin (ΔI _Amil) were quantified and presented as mean ± 95% CI (right panel). (b) Typical blots obtained using antibodies against the Thr^346/356/366‐phosphorylated form of the protein encoded by N‐myc downstream regulated gene 1 (upper panel, NDRG1‐P‐Thr^346/356/366) and the equivalent full length protein (lower panel, T‐NDRG1). Pooled data (right‐hand panels) from the entire series of experiments showing (mean ± 95% CI, n = 6) the effects of insulin (20 nM, 1 hr) upon the abundance of NDRG1‐P‐Thr^346/356/366 (upper) and total NDRG1 (lower) in control and TSA‐treated (1 μM, 2 hr) cells. *P<0.05, significantly different as indicated; two‐way ANOVA and Tukey's post hoc test